Synergistic Effect of rhBMP-2 Protein and Nanotextured Titanium Alloy Surface to Improve Osteogenic Implant Properties

One of the major limitations during titanium (Ti) implant osseointegration is poor cellular interactions at biointerface. In present study, combined effect recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) and nanopatterned Ti6Al4V fabricated with Directed irradiation synthesis (DIS) investigated in vitro. This environmentally-friendly plasma uses ions to create self-organized nanostructures on surfaces. Nanocones (≈36.7 nm DIS 80°) thinner nanowalls (≈16.5 60°) were depending incidence angle observed via scanning electron microscopy. All samples have a similar crystalline structure wettability, except for sandblasted/acid-etched (SLA) acid-etched/anodized (Anodized) which are more hydrophilic. Biological results revealed that viability adhesion properties (vinculin expression cell spreading) 80° BMP-2 those polished BMP-2, yet we filopodia (≈39 filopodia/cell) compared other (<30 filopodia/cell). increased alkaline phosphatase activity all samples, tending be higher 80°. Moreover, mineralization studies, Anodized formation calcium deposits (>3.3 fold) BMP-2. Hence, this study shows there synergistic surface modification improving Ti biological could applied bone implants treat disease.